Uji Aktivitas Ekstrak Etanol Daun Salam (Syzygium polyanthum) Terhadap Pertumbuhan Bakteri Methicilin-Resistant Staphylococcus aureus
Keywords:
Antibacterial, Ethanol Extract, Bay Leaf, Methicillin-Resistant staphylococcus aureus.Abstract
Bay leaf (Syzygium polyanthum) is a family of Myrtaceae, which has been widely used as a spice in the kitchen, it turns out that it can be used as a traditional medicine to treat various diseases such as diabetes mellitus, hypertension, diarrhea, cholesterol, gastritis, and gout. Bay leaf (Syzygium polyanthum) can be used as a traditional medicine to maintain health and prevent various diseases because it contains alkaloid compounds, tannins, flavonoids, essential oils, and saponins. Methicillin-Resistant staphylococcus aureus (MRSA) is a cause of bacterial infections such as bacteremia, pneumonia, and surgical wound infections. Methicillin-Resistant Staphylococcus aureus can cause various complications, often in the bloodstream, joints, lungs, bones, and heart. This study was conducted with the aim of knowing the antibacterial activity of the ethanolic extract of bay leaves (Syzygium polyanthum) with concentrations of 5%, 10%, 15%, 20%, and 25% against the growth of Methicillin-Resistant staphylococcus aureus bacteria. This research method uses an experimental method, namely to determine the antibacterial activity of ethanol extract from bay leaves (Syzygium polyanthum) against the growth of Methicillin-Resistant staphylococcus aureus bacteria. This research used 96% ethanol as solvent. The test was carried out through several stages including material collection, preparation of Simplicia, manufacture of ethanol extract from bay leaves by maceration, and testing of antibacterial activity against Methicillin-Resistant staphylococcus aureus bacteria. Based on the results of phytochemical screening tests, the thick extract of bay leaf contains a class of alkaloids, flavonoids, glycosides, saponins, and tannins. The results of the antibacterial activity test of thick bay leaf extract against Methicillin-Resistant staphylococcus aureus bacteria had an average inhibitory activity at concentrations of 5% (6.55 mm), 10% (7.5 mm), 15% (8 mm), 20 % (8.8 mm) and 25% (11.06 mm).
Downloads
References
Abdul, Rokhman, and Qorriuyu Nana. 2020. “Kejadian Stunting Pada Anak Usia Prasekolah (3-5 Tahun) Berdasarkan Status Sosial Ekonomi Dan Penyakit Infeksi.” Jurnal Kesehatan 9(2): 73–85.
Firdaus, Tazkiyatul. 2014. “Efektivitas Ekstrak Bawang Dayak (Eleutherine Palmifolia) Dalam Menghambat Pertumbuhan Bakteri Staphylococcus Aureus.”
Habibi, Ahmad Ikhwan, R Arizal Firmansyah, and Siti Mukhlishoh Setyawati. 2018. “Skrining Fitokimia Ekstrak N-Heksan Korteks Batang Salam (Syzygium Polyanthum).”Indonesian Journal of Chemical Science 7(1):1-4.
Harismah, Kun. 2017. “Pemanfaatan Daun Salam (Eugenia Polyantha) Sebagai Obat Herbal Dan Rempah Penyedap Makanan.” Warta Lpm 19(2): 110–18.
Hidayati, Mutia Devi, Taslim Ersam, Kuniyoshi Shimizu, and Sri Fatmawati. 2017. “Antioxidant Activity of Syzygium Polyanthum Extracts.” Indonesian Journal of Chemistry 17(1): 49–53.
Inayatullah, Seila. 2012. “Efek Ekstrak Daun Sirih Hijau (Piper Betle L.) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus.”
Konoralma, Ketrina. 2019. “Identifikasi Bakteri Penyebab Infeksi Nosokomial Di Rumah Sakit Umum GMIM Pancaran Kasih Manado.” Kesmas 8(1).
Kusnadi, Anatasia Melinda. 2014. “Efek Antimikroba Ekstrak Etanol Daun Salam (Syzygium Polyanthum) Terhadap Staphylococcus Aureus Dan Pseudomonas Aeruginosa Secara In Vitro.”
Maida, Surah, and Kinanti Ayu Puji Lestari. 2019. “Aktivitas Antibakteri Amoksisilin Terhadap Bakteri Gram Positif Dan Bakteri Gram Negatif.” Jurnal Pijar Mipa 14(3): 189–91.
Mantasyia, Ananda Anggi Amelia. 2020. “Aktivitas Antibakteri Ekstrak Daun Salam (Syzygium Polyanthum) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus.”
Nugraha, Aditya Cahya, Agung Tri Prasetya, and Sri Mursiti. 2017. “Isolasi, Identifikasi, Uji Aktivitas Senyawa Flavonoid Sebagai Antibakteri Dari Daun Mangga.” Indonesian Journal of Chemical Science 6(2): 91–96.
Pangidoan, Vebio Romatua. 2017. “Uji Evektifitas Ekstrak Daun Salam (Syzygium Polyanthum) Terhadap Staphylococcus Aureus.”
Pratiwi, Rina Hidayati. 2017. “Mekanisme Pertahanan Bakteri Patogen Terhadap Antibiotik.” Pro-Life 4(3): 418–29.
Razak, Abdul, Aziz Djamal, and Gusti Revilla. 2013. “Uji Daya Hambat Air Perasan Buah Jeruk Nipis (Citrus Aurantifolia s.) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro.” Jurnal Kesehatan Andalas 2(1): 5–8.
Safitri, Awalia. 2017. “Dampak Infeksi Bakteri Gram Positif (Staphylococcus Aureus & S. Epidermidis) Terhadap Patogenesis Sepsis Berdasarkan Jumlah Sel Yang Terapoptosis Pada Hewan Coba Rattus Norvegicus.”
Samudra, Arum. 2014. “Karakterisasi Ekstrak Etanol Daun Salam (Syzygium Polyanthum Wight) Dari Tiga Tempat Tumbuh Di Indonesia.”
Saputri, Andri Wahyu. 2019. “Uji Antimikroba Ektrak Daun Salam (Syzygium Polyanthum) Terhadap Pertumbuhan Bakteri Escherichia Coli.”
Siregar, Rahmad Syukur et al. 2021. “Studi Literatur Tentang Pemanfaatan Obat Tradisional.” In Scenario (Seminar of Social Sciences Engineering and Humaniora), , 385–91.
Susanti, Sulis. 2016. “Pemetaan Penyakit Pneumonia Di Provinsi Jawa Timur.” Jurnal Biometrika dan Kependudukan 5(2): 117–24.
Ulfa, Lutfiana. 2019. “Penyebab Dan Dampak Penyakit ISPA.”
Wahjono, Hendro. 2007. “Peran Mikrobiologi Klinik Pada Penanganan Penyakit Infeksi.”
