EVALUATION OF PLATELET RICH PLASMA (PRP) PREPARATION PROCEDURE
Article Sidebar
Main Article Content
Abstract
The success of PRP therapy in repairing tissue damage is influenced by the PRP preparation procedure. Currently, there’s no standardization of PRP preparation procedures, and various techniques are used, such as the use of anticoagulants and different centrifugation speeds. This study aimed to evaluating the PRP preparation procedures based on the centrifugations steps (single and double centrifugation) and the use of anticoagulants variation (sodium citrate, EDTA and ACD-A). This study was an experimental study and used blood samples from respondents. The selected respondents must meet inclusion and exclusion criteria. The treatment groups in this study were the single centrifugation group and the double centrifugation group. Each group will be divided into 3 subgroups with different anticoagulant usage (sodium citrate, EDTA and ACD-A). Statistical analysis results showed a significant difference in the mean platelet count in the sodium citrate , EDTA, ACD-A groups with single and double centrifugation steps. Evaluation of platelet preparation procedures in this study, a higher platelet count was obtained, specifically in the sodium citrate group (494 x 103 cells/µL), EDTA group (829.4 x 103 cells/µL), and ACD-A group (607.1 x 103 cells/µL), compared to single centrifugation in the sodium citrate group (354.8 x 103 cells/µL), EDTA group (408.1 x 103 cells/µL), and ACD-A group (390.6 x 103 cells/µL). The highest platelet count in PRP was achieved with the preparation procedure using EDTA as the anticoagulant with double centrifugation. Further research is necessary to evaluate PRP preparation procedures regarding the concentration of growth factors present in PRP.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Syarat yang harus dipenuhi oleh Penulis sebagai berikut:
Â
- Penulis menyimpan hak cipta dan memberikan jurnal hak penerbitan pertama naskah secara simultan dengan lisensi di bawah Creative Commons Attribution License yang mengizinkan orang lain untuk berbagi pekerjaan dengan sebuah pernyataan kepenulisan pekerjaan dan penerbitan awal di jurnal ini.
- Penulis bisa memasukkan ke dalam penyusunan kontraktual tambahan terpisah untuk distribusi non ekslusif versi kaya terbitan jurnal (contoh: mempostingnya ke repositori institusional atau menerbitkannya dalam sebuah buku), dengan pengakuan penerbitan awalnya di jurnal ini.
- Penulis diizinkan dan didorong untuk mem-posting karya mereka online (contoh: di repositori institusional atau di website mereka) sebelum dan selama proses penyerahan, karena dapat mengarahkan ke pertukaran produktif, seperti halnya sitiran yang lebih awal dan lebih hebat dari karya yang diterbitkan. (Lihat Efek Akses Terbuka).
References
Abdulla AK, Rebai T, Al-Delemi DHJ. 2022. Testicular Injection of Autologous Platelet-Rich Plasma (PRP) to Enhance the Sperm Parameters in Rabbit. Eurasian Medical Research Periodical. 68(4): 113-121.
Aizawa H, Kawabata H, Sato A, Masuki H, Watanabe T, Tsujino T, Isobe K, Nakamura M, Nakata K, Kawase T. 2020. A comparative study of the effects of anticoagulants on pure platelet-rich plasma quality and potency. Biomedicines, 8(3), 1–14.
Alam M. 2022. Platelet Rich Plasma (PRP) for scalp hair loss. Journal of Pakistan Association of Dermatologists. 32(4):715-718.
Alkady OH, Ahmed RS, Abdou DM, Rezk SM, Elgabry S. 2020. Platelet-rich plasma preparation using three different centrifugation methods: A comparative study. Egypt J Lab Med. 32(2): 40-44.
Anitua E, Prado R, Troya M, Zalduendo M, de la Fuente M, Pino A, Muruzabal F, Orive G. 2016. Implementation of a more physiological plasma rich in growth factor (PRGF) protocol: Anticoagulant removal and reduction in activator concentration. Platelets, 27(5):459-66.
Anwari F., Wahyuni KI., Charisma AM., Oktaviani E. 2020. Lama Penyimpanan Darah Terhadap Jumlah Trombosit Pasien DBD Di RS X Mojokerto. Jurnal Analis Laboratorium Medik. 5(2): 17-22.
Astuti LA, Hatta M, Oktawati S, Chandra MH, Djais AI. 2018. effect of centrifugation speed and duration of the quantity of platelet rich plasma (PRP). 10.13140/RG.2.2.26300.59524.
Bhatia A, Ramya BS, Biligi D, Prasanna B. 2016. Comparison of different methods of centrifugation for preparation of platelet-rich plasma (PRP). Indian Journal of Pathology and Oncology. 3 (4): 535-539.
Chen Z, Deng Z, Ma Y, Liao J, Li Q, Li M, Liu H, Chen G, Zeng C, Zheng Q. 2018. Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis. Journal of Visualized Experiments. 4 (143):1-5.
Chorążewska M, Piech P, Pietrak J, Kozioł M, Obierzyński P, Maślanko M, Wilczyńska K, Tulwin T, Łuczyk R. 2017. The use of platelet-rich plasma in anti-aging therapy (owerview). Journal of Education, Health and Sport. 7(11):162-175.
Clarissa S, Nugraha J, Ruddy T. 2019. Perbedaan Jumlah Trombosit Platelet Rich Plasma Yang Menggunakan Tabung Natrium Sitrat Dan Tabung ACD-A. Jurnal Widya Medika. 5 (1): 24-34.
de Pochini AC, Antonioli E, Bucci DZ, Sardinha LR, Andreoli CV, Ferretti M, et al. 2016. Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns. Einstein (Sao Paulo) 14, 391–397.
do Amaral RJ, da Silva NP, Haddad NF, Lopes LS, Ferreira FD, Filho RB, Cappelletti PA, de Mello W, Cordeiro-Spinetti E, Balduino A. 2016. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro. Stem Cells Int. 2016:7414036.
El Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA. 2017. Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab. 08 (03).
Hasan I, Kumar P. 2020. Platelet Rich Plasma (P.R.P) Treatment: A View. International Journal of Clinical Nursing. 1(1):34-47.
Hua L, Lai G, Zhenjun L, Guie M. [The study of anticoagulants selection in platelet-rich plasma preparation]. Zhonghua Zheng Xing Wai Ke Za Zhi. 2015 Jul;31(4):295-300. Chinese. PMID: 26665933.
Karina, Wahyuningsih KA, Sobariah S, Rosliana I, Rosadi I, Widyastuti T, Afini I, Wanandi SI, Soewondo P, Wibowo H, Pawitan JA. 2019. Evaluation of platelet-rich plasma from diabetic donors shows increased platelet vascular endothelial growth factor release. Stem cell investigation. 6, 43.
Kasper DL, Fauci AS, Hauser SL, Longo DL, Jameson J, Loscalzo J. Bleeding and thrombotic disorders (Eds.). 2016. Harrison's Manual of Medicine, 19e. McGraw Hill. https://accessmedicine.mhmedical.com/content.aspx?bookid=1820§ionid=127555232
Kutlu B, Tiˇglı Aydın RS, Akman AC, Gümü¸sderelioglu M, Nohutcu RM. 2013. Platelet-rich plasma-loaded chitosan scaffolds: preparation and growth factor release kinetics. J. Biomed. Mater. Res. B. Appl. Biomater. 101, 28–35.
Lansdown DA, Fortier LA. 2017. Platelet-Rich Plasma: Formulations, Preparations, Constituents, and Their Effects. Oper Tech Sports Med. 25:7-12.
Rachita D, Sukesh MS. 2014. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author's Perspective. Surgery Aest Cutaneous J. 2014;7(4):189-95
Rattanasuwan K, Rassameemasmaung S, Kiattavorncharoen S, Sirikulsathean A, Thorsuwan J, Wongsankakorn W. 2018. Platelet-rich plasma stimulated proliferation, migration, and attachment of cultured periodontal ligament cells. Eur J Dent. 12(4):469-474.
Rizal DM, Puspitasari I, Yuliandari A. 2020. Protective Effect of PRP against Testicular Oxidative Stress on D-galactose Induced Male Rats. AIP Conf Proc. 2020;2260.
Rofi’i, Utomo DN. 2012. Effect Of Making Method Of Platelet Rich Plasma On Platelet And Growth Factor (PDGF-BB & TGF-β1) Consentration. Media Orthopaedi. 1(1).
Shin HS, Woo HM, Kang BJ. 2017. Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma. BMC veterinary research, 13(1), 198.
Singh S. 2018. Comparative (quantitative and qualitative) analysis of three different reagents for preparation of platelet-rich plasma for hair rejuvenation. J. Cutan. Aesthet. Surg. 11:127.
Yuliandari A, Hartuti Y, Tomahu DYP, Hartini H. 2022. The Effectiveness of PRP on Reducing Blood Glucose Levels in Diabetic Mice. Jurnal Analis Laboratorium Medik. 7(2): 60–65